Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: A schematic illustration of the preparation of MSCs-laden gelatin methacrylate/silk fibroin (GelMA/SF, SG) hydrogel with the incorporation of PRP for treating KOA to reconstruct cartilage.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques:

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: The MSCs proliferation after loaded in SG hydrogels and cultured for 72 h, cells cultured without hydrogel as a control group. Laser scanning confocal microscope (LSCM) images of MSCs loaded in SG-1 ( A , B , and C ), SG-2 (D, E, and F), and SG-3 ( G , H , and I ) hydrogels, and without hydrogels ( J , K , and L ) cultured for 72 h, images ( A , D , G , and J ) displayed that cell nucleus stained using DAPI as shown in blue color, images ( B , E , H , and K ) displayed that cytoskeleton stained using Phalloidin-FITC as shown in green color, and image ( C , F , I , and L ) was merged from DAPI, and Phalloidin-FITC stained images, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Cell Culture, Control, Microscopy, Staining

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: Cell viability of MSCs after loaded in SG hydrogels and cultured for different days. * p < 0.05, and ** p < 0.01.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Cell Culture

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: Optical micrographs of ( A-H ) H&E and ( I–P ) Masson staining slices of tissues after SG hydrogels ( C, D, K, and L ), SG hydrogels incorporated with PRP ( E, F, M, and N ), and MSCs-laden SG hydrogels incorporated with PRP ( G, H, O, and P ) were intra-articular injected into SD rats for 8 weeks, respectively, the SD rats without hydrogels intervention as the control group ( A, B, I, and J ).

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Staining, Injection, Control

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: The obtained scores for SG hydrogel, SG hydrogel with PRP, MSCs-loaded hydrogel with PRP, and control group using Mankin Scoring system. * p < 0.05, and ** p < 0.01.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Control

Journal: Biofabrication

Article Title: BoneMA—synthesis and characterization of a methacrylated bone-derived hydrogel for bioprinting of in-vitro vascularized tissue constructs

doi: 10.1088/1758-5090/abb11f

Figure Lengend Snippet: Representative fluorescence microscopy image demonstrating the range of patterns and geometries achieved by printing HMSC-laden BoneMA with a DLP printer (Ember). The cells were stained with F-Actin (green) after printing to enable confirmation of the shape and structure of the printed constructs through fluorescence microscopy. (Scale bar – 750 μm)

Article Snippet: To that end, human dental pulp stem cells (HDPSC) and human mesenchymal stem cells (HMSC) were used to assess cytocompatibility and bioprintability, while green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) (cAP0001GFP, Angio-proteomie, USA) were employed to investigate the vasculogenic potential of the synthesized biomaterial.

Techniques: Fluorescence, Microscopy, Staining, Construct

Journal: Pharmaceutics

Article Title: Chitin Nanofibril Application in Tympanic Membrane Scaffolds to Modulate Inflammatory and Immune Response

doi: 10.3390/pharmaceutics13091440

Figure Lengend Snippet: Bar graphs displaying the AlamarBlue ® assay results, indicated as dye reduction percent, of hMSCs differentiated into fibroblasts for 8 days on PEOT/PBT and PEOT/PBT/(CN/PEG 50:50) scaffolds. Data are expressed as mean ± standard deviation ( n = 3; ** p < 0.01; *** p < 0.001).

Article Snippet: Human mesenchymal stromal cells (hMSCs) were supplied by Merck Millipore S.A.S. (Burlington, MA, USA).

Techniques: Alamar Blue Assay, Standard Deviation

Journal: Pharmaceutics

Article Title: Chitin Nanofibril Application in Tympanic Membrane Scaffolds to Modulate Inflammatory and Immune Response

doi: 10.3390/pharmaceutics13091440

Figure Lengend Snippet: SEM micrographs of hMSCs differentiated into fibroblasts on ( a , c , e ) PEOT/PBT and ( b , d , f ) PEOT/PBT/(CN/PEG 50:50) scaffolds: ( a , b ) SEM micrographs imaging elongated cells adhered on the surface of the fiber meshes. Scale bar: 50 μm, high voltage (HV) 10 kV, magnification 1600×; ( c , d ) fluorescence micrographs revealing f -actin with phalloidin (green) and cell nuclei with DAPI (blue) staining; and fluorescence micrographs revealing collagen type I (green) immunofluorescence and DAPI (blue) staining. Scale bar: 100 μm, original magnification 100×.

Article Snippet: Human mesenchymal stromal cells (hMSCs) were supplied by Merck Millipore S.A.S. (Burlington, MA, USA).

Techniques: Imaging, Fluorescence, Staining, Immunofluorescence